Frequently Asked Questions

We do not report any communications in this regard because, as stated in a sentence of the Advertising Self-Regulation Jury, the words “stop the animal tests guaranteed by LAV” and “not tested on animals” are certainly in contrast with the Code (EU Regulation no. 355 of 10.07.2013), as they can lead the consumer to understand that other cosmetic products experiment on animals, when this is prohibited by law. According to the Jury, the reference to LAV is also irrelevant, as the message is decoded not as an ideological adhesion to the general proposal to ban animal tests even outside the cosmetic sector, but as a specific merit of the product.

Advertising Self-Regulation Jury – judgment N-94/2016 of 14.02.2017
Go to the website

The total ban on testing and marketing cosmetic ingredients and products tested on animals came into force all over the European Union on 11 March 2013 with the new cosmetic regulations No. 1223/2009.  After a long process of gradual bans that lasted almost 20 years, the official NO to all tests on animals in the cosmetic sector has finally come into being.

Bios Line does not and never did carry out tests on animals (or on raw materials or finished products – Law 713/86 and subsequent modifications).To guarantee quality, effectiveness and product safety for customers, Bios Line uses methods other than tests on animals, carrying out in vitro or dermatological tests in co-operation with the best Italian universities.This commitment is expressed also through the concrete support of the research for methods that do not include animals in safety and toxicity tests promoted by the Fund for the Replacement of Animals in Medical Experiments (FRAME – Go to Official Website

As regards the absence of nickel in cosmetic products, it is only fair to clarify that it is virtually impossible to completely avoid the presence of this mineral, as it is contained in trace amounts in water and in the steel mixers where the products are prepared. Precisely for this reason, Bios Line has chosen to promote its cosmetic products as “Nickel Tested”.
This means that every production lot of BiosLine Nickel Tested cosmetic products undergoes analysis for nickel.

In this particular period, because of hormone levels that are established during breastfeeding, cellulite becomes more evident and it is very difficult to lose weight: prolactin, in fact, leads to considerable fluid retention, a prerequisite for the production of milk. Generally, experts advise against starting a low-calorie diet and/or exercise program aimed at weight loss while a woman is breast feeding, because milk production could be affected.
And also as regards cosmetic treatment and food supplements, for the same reasons mentioned above, I advise you to defer their use until the end of breastfeeding: anti-cellulite/slimming products always result in drainage of the fluids present in the tissues, which is counterproductive for milk production.

Due to the particular level of certain hormones during pregnancy, a woman’s skin becomes more sensitive and likely to show sensitization reactions.
As a consequence, products such as anti-cellulite* ones, which stimulate the skin, could cause redness and itching.
I would suggest Cell-Plus Crema Rassodante with FRV be applied on the stomach, hips and bust to prevent the appearance of stretch marks.
*skin appearance
Furthermore, bear in mind that it is quite normal that, during pregnancy and breastfeeding, fluid retention in the tissues increases due to hormones. It would actually be counterproductive to undergo treatment of any kind to reduce the volume of fluid in the tissues: women need to accumulate fluid for the continuous replacement of amniotic fluid and, later on, to produce milk.

The hair dye should be applied also on the length and tips but always remember that “colour-does-not-lighten-colour”. That is, if you have brown hair and want to achieve a blond colour, you need to do it step by step and through many applications (sometimes lightening treatments are needed, too) before reaching the wished result.

In case of already dyed hair, apply the colour only on the roots; during the last 10-15 minutes distribute the remaining mixture on length and tips.
If you are using a BioKap Nutricolor Delicato Rapid hair dye, apply it on the roots first and then on the length, paying attention not to exceed the leave-on time stated on the pack.
In case of natural, therefore undyed, hair, apply the mixture on the length and tips first, then on the roots, always following the instructions you can find in the internal leaflet.

Hair dyes can cause serious allergy reactions, for this reason we recommend strictly following the instructions. As reported on the packaging, do a sensitivity test 48 hours before each application. If you wish to know how to carry out the test, read the instructions on the internal leaflet carefully and also the FAQ section “Before applying the hair dye  – How to do the sensitivity test”.

In case of an allergic reaction during application, as for instance intense itching, skin burning or skin rash, immediately stop using the product and immediately rinse with warm water. In this case, before applying a new dye, consult a Dermatologist.

All products in the BioKap Nutricolor range are “Nickel Tested”, (link to the question above) as shown on each pack.
This means that the finished product from each production lot is subjected to analysis for nickel. In any case, it is necessary to carry out a sensitivity test 48 hours before using the product.

Yes, it is possible to apply BioKap Nutricolor hair dyes on already-dyed hair. If you need to just touch up your roots, choose a colour similar to the one you already have.  If instead you want to change your current look, choose a colour darker than your original one.  If the percentage of grey hair is high, apply a colour that is one tone darker than your hair in order to get a perfect coverage. Like all permanent hair dyes, we recommend waiting at least 4/5 weeks between one application and the other.

It is first necessary to perform the patch test 48 hours before each application. The dye can be applied but we cannot guarantee the colour result, as the previous treatment can alter the final colour of the first application compared to the desired one. Subsequent applications, one month apart, can help obtain the desired colour. We nevertheless recommend choosing shades that do not have red hues and testing a lock behind your neck to see the final result.

If the final result is a colour one or two shades darker than your natural one, you can lighten your hair using BioKap Nutricolor Delicato Lightening Cream. If the final result is a colour one or two shades lighter than your natural one, you can apply a darker shade of dye. In any case, before proceeding you should wait 4-5 weeks from the first hair dye application. If the final result is a colour much lighter or much darker than your natural one, you should go to the hairdresser.

The Lightening Cream should be applied in the same way as BioKap Nutricolor Hair Dyes, with the only following differences:

  • – to lighten the hair by 1-2 shades: apply over the entire length, leaving it in for 30/40 minutes;
  • – for highlights: apply to small locks using the special applicator, wrap in aluminium foil and leave in for 30 minutes.

Some guidelines: if your natural colour is:

  • dark blond: your hair will become light blond;
  • brown: your hair will become light brown;
  • red: be careful because your hair might turn to orange;
  • dark brown/black: it is not advisable to apply the product.

For a correct application, the development time is 35 minutes altogether. We recommend applying the dye first on the regrowth, leaving it in for approximately 20 minutes, and then distributing it along the length, leaving it in for another 15 minutes.

BioKap Nutricolor Delicato Rapid hair dyes have a leave-on time of 10 minutes.The leave-on time is calculated from the moment you finish the colour application. The whole colour application should take maximum 5 minutes. If it takes longer, reduce the leave on time accordingly. For example, if you need 1-2 minutes more for applying the hair dye, the total leave-on time should be 8-9 minutes. We recommend starting from the roots and during the last 2-3 minutes applying the dye on the hair lengths and tips.
We do not recommend exceeding the 10 minutes leave-on time as the final colour may be darker than expected.

It is usually best to wait 5-6 weeks before repeating the application, but time may vary depending on how fast your hair grows.

If you cannot find the colour that better suits you among  Nutricolor and Nutricolor Delicato ranges, it is possible to mix the different colours among them.
The BioKap Nutricolor Delicato RAPID hair dyes can only be mixed among them, as they have a 10-minute application time.

We remind you that any residue of hair dye that has been already mixed and is ready for use cannot be stored and used later. It is also essential to perform the patch test 48 hours before each application. This must be done, even if you have already used a dyeing product from this or another brand, as individual sensitivity to dyes may change over time.

A preliminary test should be carried out to check the final result. Depending on the final colour you wish to obtain, you can proceed by mixing different amounts of the chosen colours in the same bottle of Nutrifix Colour Revealer.
We remind you not to mix hair dyes with different leave-on time.

After a few washes the dye stains will disappear. However, in order to avoid such inconvenience, you can apply the protective cream supplied in the pack, or another moisturizing cream, to your hairline and around your forehead before dye application.

In this case, rinse immediately with plenty of water.

The product must NOT be used to dye eyelashes, eyebrows, beards and moustaches. It has been specifically formulated for hair colouring.

BioKap hair dyes cover grey hair from the first application. For best results we recommend the following:

  • with less than 50% grey hair: use a shade similar to your own to maintain your colour;
  • with more than 50% grey hair, use a shade darker than your own to achieve your colour.

Hair colouring products must not be used if the user:

  • has had a previous reaction to hair dyes;
  • has a sensitive, irritated or damaged scalp or rashes on the face;
  • has had a reaction to a temporary black henna tattoo.

In addition, these products are not intended to be used on people under the age of 16.

Mix a small amount of BioKap Nutricolor Colouring Cream with an equal amount of Nutrifix Colour Revealer. As a container you can use a lid, a paper cup or a coffee cup.
Using a cotton bud, apply a small amount of the product behind the ear or in the crook of the elbow, enough to cover an area equal in size to about 1 cm square.
Repeat it two or three times, letting it dry in between. Close the Colouring Cream tube and the Nutrifix Colour Revealer bottle carefully, then wait 48 hours without washing, covering or touching the area.
If itching, redness or other skin disorders occur during this period of time, do not use the product and consult a dermatologist before using any other colouring product.
The patch test is a reasonable precaution that can reduce, but not cancel, the risk of an allergic reaction, even in the event of a negative result. If, after this period, no itching, redness or swelling is evident, proceed with the application of BioKap Nutricolor Hair Dyes.

Not without performing the patch test. Each hair dye is different and it is essential to perform the patch test 48 hours before each application. This must be done, even if you have already used a colouring product from this or another brand, as individual sensitivity to dyes may change over time.

Regarding the use of our hair dyes during pregnancy, we recommend that you follow your gynaecologist advice.
In fact, although the ingredients are selected to ensure high skin tolerance, we must consider that during pregnancy skin is more sensitive. Therefore, there is an increased risk of sensitization or allergy, even in women who have never experienced this type of problem.
For this reason, we do not recommend using any hair dyes in the first three months of pregnancy and, for the other months, we suggest that you consult your gynaecologist: some recommend the use of natural dyes from the 4th month onwards, while others only after pregnancy and breastfeeding.

No. 3-month cycles are recommended, with a 1-2 months’ pause. We always recommend to take supplements in cycles, also not to become psychologically dependant.

No, Ansistop is a supplement and, as such, is not addictive.

No, there are no safety data on the use of Magnolia/L-Theanine during pregnancy, therefore the use of Ansistop during pregnancy is not recommended.

From the age of 14. Under the age of 14, VitaCalm Buonanotte Gocce is recommended, 40 drops/day, with vegetable ingredients suitable for children.

No, Ansistop gives neither drowsiness nor drops in concentration.

Yes, Ansistop contributes to relaxation, reducing fear and anxiety, allowing a more “relaxed” approach to the most important engagements.

Yes, we recommend 2 tablets an hour before take-off and 2 more during take-off. Ansistop contributes to relaxation without giving drowsiness or drops in concentration.

Can I take Vitacalm Ansistop together with high pressure drugs?
Yes, Ansistop is a supplement, however it must be taken separately from the drug. Thanks to the Melissa content, it has a general relaxing effect. We recommend you take it 4 hours after/before the drug.

Yes, there is no interaction between the two products.

Yes, Ansistop causes no drowsiness or drops in concentration.


Stability, chemical/physical and qualitative checks with the use of the most advanced instruments.


ELISA tests are carried out for the search for gluten and that for the search for nickel.


The goal is to preserve and enhance the purity and effectiveness of the active ingredients.


Professionalism, enthusiasm and passion are the key to the success of our products.